Assay of Chikungunya Virus in Cell Monolayers by Immunofluorescence

Chikungunya virus was quantitatively assayed by counting immunofluorescent foci after infection of BHK21/C13 cell monolayers. The speed and efficiency of virus attachment to cells were markedly enhanced when augmented by centrifugal force. By this procedure, a proportionality was obtained between th...

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Bibliographic Details
Published in:Appl Microbiol
Main Authors: Hahon, Nicholas, Hankins, William A.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1970
Subjects:
Clinical Microbiology, Virology, and Immunology
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC376656/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4908526/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/am.19.2.224-231.1970
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