Improved Cell Dispersal Method by Low-Temperature Incubation of Tissue in a Trypsin-Citrate Solution

Tissues soaked at low temperature in a trypsin-citrate solution, washed to remove enzymes, and disrupted by rapid mechanical vibrations gave yields of viable cells 1.6 to 5 times those from conventional procedures.

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Detalhes bibliográficos
Publicado no:Appl Microbiol
Main Authors: van Wezel, A. L., van Hemert, P. A., Parisius, W., Cucakovich, N. B., Macmorine, H. G.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1972
Assuntos:
Clinical Microbiology and Immunology
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC376550/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16349935/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/am.24.3.506-507.1972
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