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An efficient protocol for the purification and labeling of entire yeast septin rods from E.coli for quantitative in vitro experimentation
BACKGROUND: The detailed understanding of the functions and mechanisms of the actin and microtubuli cytoskeleton depended, besides innovative methods in live cell imaging, on the purification and labeling of its constituents. This allowed researchers to quantitatively measure filament stability, the...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3765318/ https://ncbi.nlm.nih.gov/pubmed/23889817 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-13-60 |
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