Improvements in the Growth of BHK-21 Cells in Submerged Culture

A medium is described which will support the submerged culture of BHK-21 cells to 6.5 × 10(6) to 7 × 10(6) cells/ml in volumes up to 30 liters. Shaken-flask cultures were used to determine nutrient deficiencies in depleted medium. The final medium became limited by glutamine at 6.5 × 10(6) to 7 × 10...

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Detalhes bibliográficos
Publicado no:Appl Microbiol
Main Authors: Radlett, P. J., Telling, R. C., Stone, C. J., Whiteside, J. P.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1971
Assuntos:
Metabolism and Products
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC376357/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4331766/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/am.22.4.534-537.1971
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