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Isothermal amplification method for next-generation sequencing
We report an approach for generating immobilized monoclonal templates for next- generation sequencing applications. Our isothermal amplification method is based on a template walking mechanism using a pair of low-melting temperature (Tm) solid-surface homopolymer primers and a low-Tm solution phase...
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| Hlavní autoři: | , , , , , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
National Academy of Sciences
2013
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3761594/ https://ncbi.nlm.nih.gov/pubmed/23940326 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1311334110 |
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