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Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system

The ability to artificially control transcription is essential both to the study of gene function and to the construction of synthetic gene networks with desired properties. Cas9 is an RNA-guided double-stranded DNA nuclease that participates in the CRISPR-Cas immune defense against prokaryotic viru...

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Bibliografiske detaljer
Main Authors: Bikard, David, Jiang, Wenyan, Samai, Poulami, Hochschild, Ann, Zhang, Feng, Marraffini, Luciano A.
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3753641/
https://ncbi.nlm.nih.gov/pubmed/23761437
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt520
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