Tracking Single Cells in Live Animals Using a Photoconvertible Near-Infrared Cell Membrane Label

We describe a novel photoconversion technique to track individual cells in vivo using a commercial lipophilic membrane dye, DiR. We show that DiR exhibits a permanent fluorescence emission shift (photoconversion) after light exposure and does not reacquire the original color over time. Ratiometric i...

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Detalhes bibliográficos
Main Authors: Carlson, Alicia L., Fujisaki, Joji, Wu, Juwell, Runnels, Judith M., Turcotte, Raphaël, Celso, Cristina Lo, Scadden, David T., Strom, Terry B., Lin, Charles P.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3753322/
https://ncbi.nlm.nih.gov/pubmed/23990881
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0069257
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