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Sequencing and validation of housekeeping genes for quantitative real-time PCR during the gonadotrophic cycle of Diploptera punctata

BACKGROUND: Quantitative RT-PCR (q-RT-PCR) is a powerful tool that allows for the large scale analysis of small changes in gene expression. Accurate and reliable results depend on the use of stable reference genes for normalization. However, the expression of some widely used housekeeping genes can...

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主要な著者: Marchal, Elisabeth, Hult, Ekaterina F, Huang, Juan, Tobe, Stephen S
フォーマット: Artigo
言語:Inglês
出版事項: BioMed Central 2013
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC3750588/
https://ncbi.nlm.nih.gov/pubmed/23777660
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-6-237
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