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A reproducible method to enrich membrane proteins with high-purity and high-yield for an LC-MS/MS approach in quantitative membrane proteomics
The proportionately low abundance of membrane proteins hampers their proteomic analysis, especially for a quantitative LC-MS/MS approach. To overcome this limitation, a method was developed that consists of one cell disruption step in a hypotonic reagent using liquid nitrogen, one isolation step usi...
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3749515/ https://ncbi.nlm.nih.gov/pubmed/23334993 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elps.201200503 |
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