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Size Of Gene Specific Inverted Repeat - Dependent Gene Deletion In Saccharomyces cerevisiae
We describe here an approach for rapidly producing scar-free and precise gene deletions in S. cerevisiae with high efficiency. Preparation of the disruption gene cassette in this approach was simply performed by overlap extension-PCR of an invert repeat of a partial or complete sequence of the targe...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3748122/ https://ncbi.nlm.nih.gov/pubmed/23977230 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0072137 |
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