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N-Terminal Domain of Turkey Pancreatic Lipase is Active on Long Chain Triacylglycerols and Stabilized by Colipase

The gene encoding the TPL N-terminal domain (N-TPL), fused with a His6-tag, was cloned and expressed in Pichia pastoris, under the control of the glyceraldehyde-3-phosphate dehydrogenase (GAP) constitutive promoter. The recombinant protein was successfully expressed and secreted with an expression l...

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Detalhes bibliográficos
Main Authors: Bou Ali, Madiha, Karray, Aida, Gargouri, Youssef, Ben Ali, Yassine
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3745449/
https://ncbi.nlm.nih.gov/pubmed/23977086
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0071605
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