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454 next generation-sequencing outperforms allele-specific PCR, Sanger sequencing, and pyrosequencing for routine KRAS mutation analysis of formalin-fixed, paraffin-embedded samples
Detection of KRAS mutations in archival pathology samples is critical for therapeutic appropriateness of anti-EGFR monoclonal antibodies in colorectal cancer. We compared the sensitivity, specificity, and accuracy of Sanger sequencing, ARMS-Scorpion (TheraScreen®) real-time polymerase chain reaction...
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Główni autorzy: | , , , , , , , , , , |
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Format: | Artigo |
Język: | Inglês |
Wydane: |
Dove Medical Press
2013
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Hasła przedmiotowe: | |
Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3741083/ https://ncbi.nlm.nih.gov/pubmed/23950653 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2147/OTT.S42369 |
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