Thermostable DNA Ligase-Mediated PCR Production of Circular Plasmid (PPCP) and Its Application in Directed Evolution via In situ Error-Prone PCR

Polymerase chain reaction (PCR) is a powerful method to produce linear DNA fragments. Here we describe the Tma thermostable DNA ligase-mediated PCR production of circular plasmid (PPCP) and its application in directed evolution via in situ error-prone PCR. In this thermostable DNA ligase-mediated wh...

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Hlavní autoři: Le, Yilin, Chen, Huayou, Zagursky, Robert, Wu, J.H. David, Shao, Weilan
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2013
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3738163/
https://ncbi.nlm.nih.gov/pubmed/23633530
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/dnares/dst016
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