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Quantitation of Recombinant Protein in Whole Cells and Cell Extracts with Solid-State NMR Spectroscopy

Recombinant proteins (RPs) are commonly expressed in bacteria followed by solubilization and chromatography. Purified RP yield can be diminished by losses at any step with very different changes in methods needed to try to improve yield. Time and labor can therefore be saved by first identifying the...

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Bibliografske podrobnosti
Main Authors: Vogel, Erica P., Weliky, David P.
Format: Artigo
Jezik:Inglês
Izdano: 2013
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3734946/
https://ncbi.nlm.nih.gov/pubmed/23742073
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi4007034
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