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Characterizing and harnessing antibody cross-reactivity for the immunoaffinity purification of analytes prior to multiplexed liquid chromatography-tandem mass spectrometry
BACKGROUND: Immunoassays for 1α,25-dihydroxyvitamin D [1α,25(OH)(2)D] lack specificity. We aimed to characterize the cross-reactivity of an anti-1α,25(OH)(2)D antibody using purified vitamin D metabolites and to use these data to map the chemical features of 1α,25(OH)(2)D that are important for anti...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3731945/ https://ncbi.nlm.nih.gov/pubmed/22968104 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1373/clinchem.2012.185827 |
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