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Morphology and growth of mammalian cells in a liquid/liquid culture system supported with oxygenated perfluorodecalin

Adherent A431, BHK-21, and C2C12 cells were cultured on a flexible interface formed between two immiscible liquid phases: (i) hydrophobic perfluorodecalin (PFD) and (ii) aqueous culture medium (DMEM). BHK-21 cells formed multicellular aggregates characterized by irregular shapes. A431, as well as C2...

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Bibliographic Details
Main Authors: Pilarek, Maciej, Grabowska, Iwona, Ciemerych, Maria A., Dąbkowska, Katarzyna, Szewczyk, Krzysztof W.
Format: Artigo
Language:Inglês
Published: Springer Netherlands 2013
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3730094/
https://ncbi.nlm.nih.gov/pubmed/23666427
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10529-013-1218-2
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