Expression, purification, crystallization and preliminary crystallographic analysis of spermidine acetyltransferase from Escherichia coli

The spermidine acetyltransferase (SAT) from Escherichia coli catalyses the transfer of acetyl groups from acetyl-CoA to spermidine. SAT has been expressed and purified from E. coli. SAT was crystallized by the sitting-drop vapour-diffusion method to obtain a more detailed insight into the molecular...

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Bibliographic Details
Main Authors: Niiyama, Mayumi, Sugiyama, Shigeru, Hirose, Mika, Ishikawa, Sae, Tomitori, Hideyuki, Higashi, Kyohei, Yamashita, Tomoko, Adachi, Hiroaki, Takano, Kazufumi, Murakami, Satoshi, Murata, Michio, Inoue, Tsuyoshi, Mori, Yusuke, Kashiwagi, Keiko, Matsumura, Hiroyoshi, Igarashi, Kazuei
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2013
Subjects:
Crystallization Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3729165/
https://ncbi.nlm.nih.gov/pubmed/23908034
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309113017132
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