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Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families

In this study, a protocol is described for rapid preparation of an enriched, reasonably pure fraction of nuclear proteins from the leaves of tobacco (Nicotiana tabacum), potato (Solanum tuberosum) and apple (Malus domestica). The protocol gives reproducible results and can be carried out quickly in...

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Detalhes bibliográficos
Main Authors: Sikorskaite, Sidona, Rajamäki, Minna-Liisa, Baniulis, Danas, Stanys, Vidmantas, Valkonen, Jari PT
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3728069/
https://ncbi.nlm.nih.gov/pubmed/23886449
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1746-4811-9-31
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