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Out with the Old, In with the New? Comparing Methods for Measuring Protein Degradation

Protein degradation is a key variable in controlling protein abundance in cells. Here, we compare classical methods to measure protein degradation rates with a novel GFP reporter library based method that characterizes degradation of thousands of individual proteins by flow cytometry. While no metho...

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Détails bibliographiques
Auteurs principaux: Yewdell, Jonathan W., Lacsina, Joshua R., Rechsteiner, Martin C., Nicchitta, Christopher V.
Format: Artigo
Langue:Inglês
Publié: 2011
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3727619/
https://ncbi.nlm.nih.gov/pubmed/21476986
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/CBI20110055
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