Isolation of circulating immune complexes using Raji cells. Separation of antigens from immune complexes and production of antiserum.
Raji cells were used for the isolation of complement-fixing antigen-antibody complexes from serum. Immune complexes bound to these cells were radiolabeled at the cell surface with lactoperoxidase. The complexes were then eluted from the cells with isotonic citrate buffer pH 3.2 or recovered by immun...
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| Опубликовано в:: | J Clin Invest |
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| Главные авторы: | , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
American Society for Clinical Investigation
1978
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC372683/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/659616/ https://ncbi.nlm.nih.govhttps://doi.org/10.1172/JCI109077 |
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