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Live-cell imaging of Aspergillus nidulans autophagy: RAB1 dependence, Golgi independence and ER involvement

We exploited the amenability of the fungus Aspergillus nidulans to genetics and live-cell microscopy to investigate autophagy. Upon nitrogen starvation, GFP-Atg8-containing pre-autophagosomal puncta give rise to cup-shaped phagophores and circular (0.9-μm diameter) autophagosomes that disappear in t...

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Bibliografiske detaljer
Main Authors: Pinar, Mario, Pantazopoulou, Areti, Peñalva, Miguel A.
Format: Artigo
Sprog:Inglês
Udgivet: Landes Bioscience 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3722313/
https://ncbi.nlm.nih.gov/pubmed/23722157
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/auto.24483
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