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Thiouracil Cross-Linking Mass Spectrometry: a Cell-Based Method To Identify Host Factors Involved in Viral Amplification

Eukaryotic RNA viruses are known to utilize host factors; however, the identity of these factors and their role in the virus life cycle remain largely undefined. Here, we report a method to identify proteins bound to the viral RNA during amplification in cell culture: thiouracil cross-linking mass s...

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Detalhes bibliográficos
Main Authors: Lenarcic, Erik M., Landry, Dori M., Greco, Todd M., Cristea, Ileana M., Thompson, Sunnie R.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3719794/
https://ncbi.nlm.nih.gov/pubmed/23740976
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.00950-13
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