Comparison of Pyrosequencing, Sanger Sequencing, and Melting Curve Analysis for Detection of Low-Frequency Macrolide-Resistant Mycoplasma pneumoniae Quasispecies in Respiratory Specimens

Macrolide-resistant Mycoplasma pneumoniae (MRMP) is emerging worldwide and has been associated with treatment failure. In this study, we used pyrosequencing to detect low-frequency MRMP quasispecies in respiratory specimens, and we compared the findings with those obtained by Sanger sequencing and S...

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Main Authors: Chan, Kwok-Hung, To, Kelvin K. W., Chan, Betsy W. K., Li, Clara P. Y., Chiu, Susan S., Yuen, Kwok-Yung, Ho, Pak-Leung
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2013
Fag:
Bacteriology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3719661/
https://ncbi.nlm.nih.gov/pubmed/23720793
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00785-13
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