Improved Bacterial Mutagenesis by High-Frequency Allele Exchange, Demonstrated in Clostridium difficile and Streptococcus suis

Here we show that the frequency of mutant isolation by two-step allele exchange can be improved by increasing the length of homologous DNA and the opportunity for recombination, obviating the need for counterselection markers. These principles are demonstrated in Clostridium difficile and Streptococ...

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Bibliographic Details
Main Authors: Faulds-Pain, Alexandra, Wren, Brendan W.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2013
Subjects:
Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3719504/
https://ncbi.nlm.nih.gov/pubmed/23728809
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01195-13
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