Isolation and genetic characterization of human coronavirus NL63 in primary human renal proximal tubular epithelial cells obtained from a commercial supplier, and confirmation of its replication in two different types of human primary kidney cells

BACKGROUND: Cryopreserved primary human renal proximal tubule epithelial cells (RPTEC) were obtained from a commercial supplier for studies of Simian virus 40 (SV40). Within twelve hrs after cell cultures were initiated, cytoplasmic vacuoles appeared in many of the RPTEC. The RPTEC henceforth deteri...

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Detalhes bibliográficos
Main Authors: Lednicky, John A, Waltzek, Thomas B, McGeehan, Elizabeth, Loeb, Julia C, Hamilton, Sara B, Luetke, Maya C
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2013
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3716658/
https://ncbi.nlm.nih.gov/pubmed/23805916
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1743-422X-10-213
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