Fifty Percent Tissue Culture Infective Dose Assay for Determining the Titer of Infectious Human Herpesvirus 8

We have developed a human herpesvirus 8 (HHV-8) 50% tissue culture infective dose (TCID(50)) assay using the T1H6-DC-SIGN cell line. Infection of T1H6-DC-SIGN cells with HHV-8 induces expression of β-galactosidase, which was used to determine TCID(50) levels. Validation of TCID(50) values was perfor...

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Detalhes bibliográficos
Main Authors: Nadgir, Sagar V., Hensler, Heather R., Knowlton, Emilee R., Rinaldo, Charles R., Rappocciolo, Giovanna, Jenkins, Frank J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2013
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3716109/
https://ncbi.nlm.nih.gov/pubmed/23554189
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00761-13
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