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New method of peptide cleavage based on Edman degradation

A straightforward cleavage method for N- acylated peptides based on the phenylthiohydantoin (PTH) formation is presented. The procedure could be applied to acid-stable resins, such as TentaGel HL–NH[Formula: see text] . We designed a cleavable linker that consists of a lysine residue with the [Formu...

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Main Authors: Bąchor, Remigiusz, Kluczyk, Alicja, Stefanowicz, Piotr, Szewczuk, Zbigniew
Format: Artigo
Jezik:Inglês
Izdano: Springer Netherlands 2013
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3713267/
https://ncbi.nlm.nih.gov/pubmed/23690169
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11030-013-9453-y
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