Improvement of Agrobacterium-mediated transformation of cucumber (Cucumis sativus L.) by combination of vacuum infiltration and co-cultivation on filter paper wicks

An improved method for genetic transformation of cucumber (Cucumis sativus L. cv. Shinhokusei No. 1) was developed. Vacuum infiltration of cotyledonary explants with Agrobacterium suspension enhanced the efficiency of Agrobacterium infection in the proximal regions of explants. Co-cultivation on fil...

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Detaylı Bibliyografya
Asıl Yazarlar: Nanasato, Yoshihiko, Konagaya, Ken-ichi, Okuzaki, Ayako, Tsuda, Mai, Tabei, Yutaka
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Springer Japan 2012
Konular:
Original Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3712137/
https://ncbi.nlm.nih.gov/pubmed/23874354
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11816-012-0260-1
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