A Targetron System for Gene Targeting in Thermophiles and Its Application in Clostridium thermocellum

BACKGROUND: Targetrons are gene targeting vectors derived from mobile group II introns. They consist of an autocatalytic intron RNA (a “ribozyme”) and an intron-encoded reverse transcriptase, which use their combined activities to achieve highly efficient site-specific DNA integration with readily p...

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Detalhes bibliográficos
Main Authors: Mohr, Georg, Hong, Wei, Zhang, Jie, Cui, Gu-zhen, Yang, Yunfeng, Cui, Qiu, Liu, Ya-jun, Lambowitz, Alan M.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
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Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3706431/
https://ncbi.nlm.nih.gov/pubmed/23874856
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0069032
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