Demonstration of Hepatitis C Virus RNA with In Situ Hybridization Employing a Locked Nucleic Acid Probe in Humanized Liver of Infected Chimeric Mice and in Needle-Biopsied Human Liver

Background. In situ hybridization (ISH) with high sensitivity has been requested to demonstrate hepatitis C virus (HCV) RNA in formalin-fixed, paraffin-embedded (FFPE) sections of the liver. Methods. ISH employing a locked-nucleic-acid- (LNA-)modified oligonucleotide probe and biotin-free catalyzed...

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Main Authors: Shiogama, Kazuya, Inada, Ken-ichi, Kohara, Michinori, Teramoto, Hidemi, Mizutani, Yasuyoshi, Onouchi, Takanori, Tsutsumi, Yutaka
Formato: Artigo
Idioma:Inglês
Publicado em: Hindawi Publishing Corporation 2013
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3703335/
https://ncbi.nlm.nih.gov/pubmed/23853723
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2013/249535
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