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Crystallization and preliminary X-ray diffraction analysis of a thermostable GDSL-family esterase, EstL5, from Geobacillus thermodenitrificans T2

The novel thermostable esterase EstL5 belonging to the GDSL family exhibits a unique cold-adaptation feature at low temperatures. To better understand its biochemical and enzymatic properties, recombinant EstL5 protein was purified and crystallized using the vapour-diffusion method. The EstL5 crysta...

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Detalhes bibliográficos
Main Authors: Yang, Zhenxing, Zhang, Yong, Qiu, Rui, Huang, Jing, Ji, Chaoneng
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3702323/
https://ncbi.nlm.nih.gov/pubmed/23832206
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430911301498X
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