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The Perfect Host: A Mouse Host Embryo Facilitating More Efficient Germ Line Transmission of Genetically Modified Embryonic Stem Cells

There is a continual need to improve efficiency in creating precise genetic modifications in mice using embryonic stem cells (ESCs). We describe a novel approach resulting in 100% germline transmission from competent injected ESCs. We developed an F1 mouse host embryo (Perfect Host, PH) that selecti...

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Detalhes bibliográficos
Main Authors: Taft, Robert A., Low, Benjamin E., Byers, Shannon L., Murray, Stephen A., Kutny, Peter, Wiles, Michael V.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3699516/
https://ncbi.nlm.nih.gov/pubmed/23844102
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0067826
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