Development and Evaluation of a Next-Generation Digital PCR Diagnostic Assay for Ocular Chlamydia trachomatis Infections

Droplet digital PCR (ddPCR) is an emulsion PCR process that performs absolute quantitation of nucleic acids. We developed a ddPCR assay for Chlamydia trachomatis infections and found it to be accurate and precise. Using PCR mixtures containing plasmids engineered to include the PCR target sequences,...

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Bibliografiske detaljer
Main Authors: Roberts, Chrissy h., Last, Anna, Molina-Gonzalez, Sandra, Cassama, Eunice, Butcher, Robert, Nabicassa, Meno, McCarthy, Elizabeth, Burr, Sarah E., Mabey, David C., Bailey, Robin L., Holland, Martin J.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2013
Fag:
Chlamydiology and Rickettsiology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3697714/
https://ncbi.nlm.nih.gov/pubmed/23637300
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00622-13
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