High-efficiency cloning of full-length cDNA.
A widely recognized difficulty of presently used methods for cDNA cloning is obtaining cDNA segments that contain the entire nucleotide sequence of the corresponding mRNA. The cloning procedure described here mitigates this shortcoming. Of the 10(5) plasmid-cDNA recombinants obtained per microgram o...
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| Publicado en: | Mol Cell Biol |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Taylor & Francis
1982
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC369769/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6287227/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.2.2.161-170.1982 |
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