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A Double Staining Method Using SYTOX Green and Calcofluor White for Studying Fungal Parasites of Phytoplankton

We propose a double staining method based on the combination of two fluorochromes, calcofluor white (CFW; specific chitinous fluorochrome) and SYTOX green (nucleic acid stain), coupled to epifluorescence microscopy for counting, identifying, and investigating the fecundity of parasitic fungi of phyt...

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Detalhes bibliográficos
Main Authors: Gerphagnon, Mélanie, Latour, Delphine, Colombet, Jonathan, Sime-Ngando, Télesphore
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3697587/
https://ncbi.nlm.nih.gov/pubmed/23603679
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00696-13
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