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A New Large-DNA-Fragment Delivery System Based on Integrase Activity from an Integrative and Conjugative Element

During the past few decades, numerous plasmid vectors have been developed for cloning, gene expression analysis, and genetic engineering. Cloning procedures typically rely on PCR amplification, DNA fragment restriction digestion, recovery, and ligation, but increasingly, procedures are being develop...

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Bibliografiske detaljer
Main Authors: Miyazaki, Ryo, van der Meer, Jan Roelof
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3697514/
https://ncbi.nlm.nih.gov/pubmed/23686268
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00711-13
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