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A New Large-DNA-Fragment Delivery System Based on Integrase Activity from an Integrative and Conjugative Element
During the past few decades, numerous plasmid vectors have been developed for cloning, gene expression analysis, and genetic engineering. Cloning procedures typically rely on PCR amplification, DNA fragment restriction digestion, recovery, and ligation, but increasingly, procedures are being develop...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3697514/ https://ncbi.nlm.nih.gov/pubmed/23686268 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00711-13 |
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