Permeabilization of ultraviolet-irradiated Chinese hamster cells with polyethylene glycol and introduction of ultraviolet endonuclease from Micrococcus luteus.

Chinese hamster V-79 cells were made permeable by treatment with polyethylene glycol and then incubated with a Micrococcus luteus extract containing ultraviolet-specific endonuclease activity. This treatment introduced nicks in irradiated, but not in unirradiated, deoxyribonucleic acid. The nicks re...

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Bibliographic Details
Main Authors: Yarosh, D B, Setlow, R B
Format: Artigo
Language:Inglês
Published: 1981
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC369667/
https://ncbi.nlm.nih.gov/pubmed/6965098
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