Reconstitution of functional mammalian U4 small nuclear ribonucleoprotein: Sm protein binding is not essential for splicing in vitro.

We have developed an in vitro splicing complementation assay to investigate the domain structure of the mammalian U4 small nuclear RNA (snRNA) through mutational analysis. The addition of affinity-purified U4 snRNP or U4 RNA to U4-depleted nuclear extract efficiently restores splicing activity. In t...

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Veröffentlicht in:	Mol Cell Biol
Hauptverfasser:	Wersig, C, Bindereif, A
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Taylor & Francis 1992
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC369587/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1532228/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.12.4.1460-1468.1992
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