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Screening and Improving the Recombinant Nitrilases and Application in Biotransformation of Iminodiacetonitrile to Iminodiacetic Acid

In this study, several nitrilase genes from phylogenetically distinct organisms were expressed and purified in E. coli in order to study their ability to mediate the biotransformation of nitriles. We identified three nitrilases: Acidovorax facilis nitrilase (AcN); Alcaligenes fecalis nitrilase (AkN)...

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Detalhes bibliográficos
Main Authors: Liu, Zhi-Qiang, Baker, Peter James, Cheng, Feng, Xue, Ya-Ping, Zheng, Yu-Guo, Shen, Yin-Chu
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3695085/
https://ncbi.nlm.nih.gov/pubmed/23826231
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0067197
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