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Thymidine-requiring mutants of Dictyostelium discoideum.

Two thymidine auxotrophs of Dictyostelium discoideum were isolated which improve the efficiency of in vivo DNA-specific radiolabeling. Mutant HPS400 lacked detectable thymidylate synthetase activity, required 50 micrograms of thymidine per ml, and incorporated sixfold more [3H]thymidine into nuclear...

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Publicado en:Mol Cell Biol
Autores principales: Podgorski, G, Deering, R A
Formato: Artigo
Lenguaje:Inglês
Publicado: Taylor & Francis 1984
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Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC369289/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6527688/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.4.12.2784
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