Vaccinia virus expression vector: coexpression of beta-galactosidase provides visual screening of recombinant virus plaques.
We constructed a plasmid coexpression vector that directs the insertion of a foreign gene of interest together with the Escherichia coli beta-galactosidase (beta gal) gene into the thymidine kinase (TK) locus of the vaccinia virus genome. Tissue culture cells that had been infected with vaccinia vir...
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| Udgivet i: | Mol Cell Biol |
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| Principais autores: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Taylor & Francis
1985
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC369169/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3939316/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.5.12.3403-3409.1985 |
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