Improved Orange and Red Ca(2+) Indicators and Photophysical Considerations for Optogenetic Applications

[Image: see text] We have used protein engineering to expand the palette of genetically encoded calcium ion (Ca(2+)) indicators to include orange and improved red fluorescent variants, and validated the latter for combined use with optogenetic activation by channelrhodopsin-2 (ChR2). These indicator...

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Bibliografiske detaljer
Main Authors: Wu, Jiahui, Liu, Lin, Matsuda, Tomoki, Zhao, Yongxin, Rebane, Aleksander, Drobizhev, Mikhail, Chang, Yu-Fen, Araki, Satoko, Arai, Yoshiyuki, March, Kelsey, Hughes, Thomas E., Sagou, Ken, Miyata, Takaki, Nagai, Takeharu, Li, Wen-hong, Campbell, Robert E.
Format: Artigo
Sprog:Inglês
Udgivet: American Chemical Society 2013
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3689190/
https://ncbi.nlm.nih.gov/pubmed/23452507
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/cn400012b
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