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Enhanced transformation of human cells by UV-irradiated pSV2 plasmids.

Irradiating the plasmid pSV2-gpt with UV (254 nm) doses up to 200 J m-2 caused a dose-dependent increase in the yield of Gpt+ transformants when the plasmid was introduced into human cells by calcium phosphate coprecipitation. UV doses greater than 1 kJ m-2 were required to reduce the efficiency of...

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Détails bibliographiques
Publié dans:Mol Cell Biol
Auteurs principaux: Spivak, G, Ganesan, A K, Hanawalt, P C
Format: Artigo
Langue:Inglês
Publié: Taylor & Francis 1984
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC368887/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6330533/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.4.6.1169-1171.1984
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