Non-Invasive Analysis of Recombinant mRNA Stability in Escherichia coli by a Combination of Transcriptional Inducer Wash-Out and qRT-PCR

mRNA stability is one among many parameters that can potentially affect the level of recombinant gene expression in bacteria. Blocking of the entire prokaryotic transcription machinery by addition of rifampicin is commonly used in protocols for analysis of mRNA stability. Here we show that such trea...

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Bibliographic Details
Main Authors: Kucharova, Veronika, Strand, Trine Aakvik, Almaas, Eivind, Naas, Adrian E., Brautaset, Trygve, Valla, Svein
Format: Artigo
Language:Inglês
Published: Public Library of Science 2013
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3686738/
https://ncbi.nlm.nih.gov/pubmed/23840466
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0066429
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