Technical Advance: Autofluorescence-based sorting: rapid and nonperturbing isolation of ultrapure neutrophils to determine cytokine production

The technical limitations of isolating neutrophils without contaminating leukocytes, while concurrently minimizing neutrophil activation, is a barrier to determining specific neutrophil functions. We aimed to assess the use of FACS for generating highly pure quiescent neutrophil populations in an an...

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Main Authors: Dorward, David A., Lucas, Christopher D., Alessandri, Ana L., Marwick, John A., Rossi, Fiona, Dransfield, Ian, Haslett, Christopher, Dhaliwal, Kevin, Rossi, Adriano G.
Format: Artigo
Jezik:Inglês
Izdano: Society for Leukocyte Biology 2013
Teme:
Technical Advance
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3685014/
https://ncbi.nlm.nih.gov/pubmed/23667167
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1189/jlb.0113040
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