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A comparative study of microbial diversity and community structure in marine sediments using poly(A) tailing and reverse transcription-PCR

To obtain a better understanding of metabolically active microbial communities, we tested a molecular ecological approach using poly(A) tailing of environmental 16S rRNA, followed by full-length complementary DNA (cDNA) synthesis and sequencing to eliminate potential biases caused by mismatching of...

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Bibliographic Details
Main Authors: Hoshino, Tatsuhiko, Inagaki, Fumio
Format: Artigo
Language:Inglês
Published: Frontiers Media S.A. 2013
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3684792/
https://ncbi.nlm.nih.gov/pubmed/23785366
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2013.00160
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