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Affinity purification of T7 RNA transcripts with homogeneous ends using ARiBo and CRISPR tags
Affinity purification of RNA using the ARiBo tag technology currently provides an ideal approach to quickly prepare RNA with 3′ homogeneity. Here, we explored strategies to also ensure 5′ homogeneity of affinity-purified RNAs. First, we systematically investigated the effect of starting nucleotides...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3683919/ https://ncbi.nlm.nih.gov/pubmed/23657939 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.037432.112 |
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