Evaluation of a Real-Time PCR Kit for Detecting Escherichia coli O157 in Bovine Fecal Samples

A commercially available real-time, rapid PCR test was evaluated for its ability to detect Escherichia coli O157. Both the sensitivity and specificity of the assay were 99% for isolates in pure culture. The assay detected 1 CFU of E. coli O157:H7 g(−1) in artificially inoculated bovine feces followi...

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Bibliografiske detaljer
Main Authors: Bono, James L., Keen, James E., Miller, Laura C., Fox, James M., Chitko-McKown, Carol G., Heaton, Michael P., Laegreid, William W.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2004
Fag:
Methods
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC368350/
https://ncbi.nlm.nih.gov/pubmed/15006817
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.3.1855-1857.2004
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