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Engineering of Family-5 Glycoside Hydrolase (Cel5A) from an Uncultured Bacterium for Efficient Hydrolysis of Cellulosic Substrates

Cel5A, an endoglucanase, was derived from the metagenomic library of vermicompost. The deduced amino acid sequence of Cel5A shows high sequence homology with family-5 glycoside hydrolases, which contain a single catalytic domain but no distinct cellulose-binding domain. Random mutagenesis and cellul...

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Détails bibliographiques
Auteurs principaux: Telke, Amar A., Zhuang, Ningning, Ghatge, Sunil S., Lee, Sook-Hee, Ali Shah, Asad, Khan, Haji, Um, Youngsoon, Shin, Hyun-Dong, Chung, Young Ryun, Lee, Kon Ho, Kim, Seon-Won
Format: Artigo
Langue:Inglês
Publié: Public Library of Science 2013
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3681849/
https://ncbi.nlm.nih.gov/pubmed/23785445
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0065727
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