Yeast pre-mRNA splicing requires a minimum distance between the 5' splice site and the internal branch acceptor site.

We have generated several deletions within the intron of a yeast actin gene construct which have lead to different splicing efficiencies as measured by Northern blot (RNA blot) and primer extension analyses. Our data especially demonstrate that a minimum distance from the 5' splice site to the...

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Bibliographic Details
Main Authors: Thompson-Jäger, S, Domdey, H
Format: Artigo
Language:Inglês
Published: 1987
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC368070/
https://ncbi.nlm.nih.gov/pubmed/3323885
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